Comparison of Post-injection Site Pain Between Technetium Sulfur Colloid and Technetium Tilmanocept in Breast Cancer Patients Undergoing Sentinel Lymph Node Biopsy.

BackgroundNo prior studies have examined injection pain associated with Technetium-99m Tilmanocept (TcTM).MethodsThis was a randomized, double-blinded study comparing postinjection site pain between filtered Technetium Sulfur Colloid (fTcSC) and TcTM in breast cancer lymphoscintigraphy. Pain was evaluated with a visual analogue scale (VAS) (0-100 mm) and the short-form McGill Pain Questionnaire (SF-MPQ). The primary endpoint was mean difference in VAS scores at 1-min postinjection between fTcSC and TcTM. Secondary endpoints included a comparison of SF-MPQ scores between the groups at 5 min postinjection and construction of a linear mixed effects model to evaluate the changes in pain during the 5-min postinjection period.ResultsFifty-two patients underwent injection (27-fTcSC, 25-TcTM). At 1-min postinjection, patients who received fTcSC experienced a mean change in pain of 16.8 mm (standard deviation (SD) 19.5) compared with 0.2 mm (SD 7.3) in TcTM (p = 0.0002). At 5 min postinjection, the mean total score on the SF-MPQ was 2.8 (SD 3.0) for fTcSC versus 2.1 (SD 2.5) for TcTM (p = 0.36). In the mixed effects model, injection agent (p < 0.001), time (p < 0.001) and their interaction (p < 0.001) were associated with change in pain during the 5-min postinjection period. The model found fTcSC resulted in significantly more pain of 15.2 mm (p < 0.001), 11.3 mm (p = 0.001), and 7.5 mm (p = 0.013) at 1, 2, and 3 min postinjection, respectively.ConclusionsInjection with fTcSC causes significantly more pain during the first 3 min postinjection compared with TcTM in women undergoing lymphoscintigraphy for breast cancer

Tillage and Nitrogen Management Effects on Nitrate-Nitrogen in the Soil Profile

A field study was conducted on Nicollet loam soils to determine the effects of tillage and nitrogen (N) management on the residual nitrate-nitrogen (NO3-N) in the soil profile. Two replications of two tillage systems (no-till and moldboard plow) and two N management practices (single application of N at the rate of 175 kg/ha and three applications totaling 125 kg/ha) were used for continuous-corn production. Soil samples were taken periodically each year to a depth of 150 cm at three locations within each of the six experimental plots (9 m apart, one along and two on either side of a tile drain running down the center of the plot). No-till and three N applications with a lower total application rate reduced the residual NO3-N in the soil profile. Regression analysis of the data showed less residual NO3-N under no-till system having a definite decreasing trend with depth. Eight years (1984-1991) of data indicate that corn yields were not affected by tillage or N management practices

cis-Acting and trans-acting modulation of equine infectious anemia virus alternative RNA splicing

AbstractEquine infectious anemia virus (EIAV), a lentivirus distantly related to HIV-1, encodes regulatory proteins, EIAV Tat (ETat) and Rev (ERev), from a four-exon mRNA. Exon 3 of the tat/rev mRNA contains a 30-nucleotide purine-rich element (PRE) which binds both ERev and SF2/ASF, a member of the SR family of RNA splicing factors. To better understand the role of this element in the regulation of EIAV pre-mRNA splicing, we quantified the effects of mutation or deletion of the PRE on exon 3 splicing in vitro and on alternative splicing in vivo. We also determined the branch point elements upstream of exons 3 and 4. In vitro splicing of exon 3 to exon 4 was not affected by mutation of the PRE, and addition of purified SR proteins enhanced splicing independently of the PRE. In vitro splicing of exon 2 to exon 3 was dependent on the PRE; under conditions of excess SR proteins, either the PRE or the 5′ splice site of exon 3 was sufficient to activate splicing. We applied isoform-specific primers in real-time RT-PCR reactions to quantitatively analyze alternative splicing in cells transfected with rev-minus EIAV provirus constructs. In the context of provirus with wild-type exon 3, greater than 80% of the viral mRNAs were multiply spliced, and of these, less than 1% excluded exon 3. Deletion of the PRE resulted in a decrease in the relative amount of multiply spliced mRNA to about 40% of the total and approximately 39% of the viral mRNA excluded exon 3. Ectopic expression of ERev caused a decrease in the relative amount of multiply spliced mRNA to approximately 50% of the total and increased mRNAs that excluded exon 3 to about 4%. Over-expression of SF2/ASF in cells transfected with wild-type provirus constructs inhibited splicing but did not significantly alter exon 3 skipping

Fertilizer and Swine Manure Management Systems: Impacts on Crop Production and Nitrate-Nitrogen Leaching with Subsurface Drainage

Nutrient losses from row-crop land can cause nonpoint source water quality problems and “impaired waters.” Nitrogen (N) losses due to nitrate (NO3) leaching cause drinking water problems and possibly increase hypoxia (low oxygen) problems in the Gulf of Mexico. Phosphorus (P) losses can cause eutrophication problems in surface waters (lakes, streams, and reservoirs) in Iowa where algal blooms decrease oxygen, kill fish, and result in murky and bad tasting water. The U.S. EPA and the Iowa Department of Natural Resources are developing nutrient criteria/standards and implementation plans to address TMDL’s (Total Maximum Daily Load) and to improve the quality of impaired waters. These plans need to be based on sound information. To address the need for information about water quality impacts from the use of swine manure as a source of nutrients (N and P), a field study was funded by the Leopold Center for Sustainable Agriculture in 1999. Properly managed, swine manure can supply N and P needed by corn and soybeans. This study is being conducted at the Northeast Research Farm on 36 one-acre plots that are instrumented to monitor subsurface drainage for continuous water quality assessment

The Incremental Garbage Collection of Processes

Key Words and Phrases: garbage collection, multiprocessing systems, processor scheduling. "lazy evaluation, "eager" evaluation. CR Categories: 3.60, 3.80, 4.13, 4.22, 4.32. This report describes research done at the Artificial Intelligence Laboratory of the Massachusetts Institute of Technology. Support for the laboratory's artificial intelligence research is provided in part by the Advanced Research Projects Agency of the Department of Defense under Office of Naval Research contract N00014-75-C-0522. This paper was presented at the AI*PL Conference at Rochester, N.Y. in August, 1977.This paper investigates some problems associated with an argument evaluation order that we call "future" order, which is different from both call-by-name and call-by-value. In call-by-future, each formal parameter of a function is bound to a separate process (called a "future") dedicated to the evaluation of the corresponding argument. This mechanism allows the fully parallel evaluation of arguments to a function, and has been shown to augment the expressive power of a language. We discuss an approach to a problem that arises in this context: futures which were thought to be relevant when they were created become irrelevant through being ignored in the body of the expression where they were bound. The problem of irrelevant processes also appears in multiprocessing problem-solving systems which start several processors working on the same problem but with different methods, and return with the solution which finishes first. This parallel method strategy has the drawback that the processes which are investigating the losing methods must be identified, stopped, and re-assigned to more useful tasks. The solution we propose is that of garbage collection. We propose that the goal structure of the solution plan be explicitly represented in memory as part of the graph memory (like Lisp's heap) so that a garbage collection algorithm can discover which processes are performing useful work, and which can be recycled for a new task. An incremental algorithm for the unified garbage collection of storage and processes is described.MIT Artificial Intelligence Laboratory Department of Defense Advanced Research Projects Agenc

The Future Role of Strategic Landpower

Recent Russian aggression in Ukraine has reenergized military strategists and senior leaders to evaluate the role of strategic Landpower. American leadership in the European theater has mobilized allies and partners to reconsider force postures for responding to possible aggression against NATO members. Although Russian revisionist activity remains a threat in Europe, the challenges in the Pacific for strategic Landpower must also be considered. At the same time, the homeland, the Arctic, climate change, and the results of new and emerging technology also challenge the application of strategic Landpower. This publication serves as part of an enduring effort to evaluate strategic Landpower’s role, authorities, and resources for accomplishing the national strategic goals the Joint Force may face in the next conflict. This study considers multinational partners, allies, and senior leaders that can contribute to overcoming these enduring challenges. The insights derived from this study, which can be applied to both the European and Indo-Pacific theaters, should help leaders to consider these challenges, which may last a generation. Deterrence demands credible strategic response options integrated across warfighting functions. This valuable edition will continue the dialogue about addressing these issues as well as other emerging ones.https://press.armywarcollege.edu/monographs/1959/thumbnail.jp